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Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici BJM
Hernández-Monjaraz,Wendy Susana; Caudillo-Pérez,César; Salazar-Sánchez,Pedro Ulises; Macías-Sánchez,Karla Lizbeth.
ABSTRACT Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic acid (copper chelator) on the activity of laccases in the intra (IF) and extracellular fractions (EF) of the wild-type and the non-pathogenic mutant strain (rho1::hyg) of F. oxysporum. The results show that laccase activity in the IF fraction of the wild and mutant strain increased with the addition of iron chelator (53.4 and 114.32%; respectively). With copper, it is observed that there is an inhibition of the activity with the addition of CuSO4 for the EF...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chelators; Cooper; Fusarium; Iron; Laccases.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500269
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Influence of iron deprivation on virulence traits of mycobacteria BJID
Pal,Rahul; Hameed,Saif; Sharma,Sharda; Fatima,Zeeshan.
ABSTRACT Novel strategies to combat the ever increasing burden of drug resistance in Mycobacterium tuberculosis (MTB) causing tuberculosis (TB) remains a global concern. The ability of MTB to sense and adapt to restricted iron conditions in the hostile environment is essential for their survival and confers the basis of their success as dreadful pathogen. The striking and clinically relevant virulence trait of MTB is its ability to form biofilms and adhere to the host cells. The present study elucidated the effect of iron deprivation on biofilm formation and cell adherence of Mycobacterium smegmatis, a non-pathogenic surrogate of MTB. Firstly, we showed that iron deprivation leads to enhanced cell sedimentation rate and altered colony morphology depicting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Morphology; Biofilm; Anti-TB drugs.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600585
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Investigations into the uptake of copper, iron and selenium by a highly sulphated bacterial exopolysaccharide isolated from microbial mats ArchiMer
Moppert, Xavier; Le Costaouec, Tinaig; Raguenes, Gerard; Courtois, Anthony; Simon-colin, Christelle; Crassous, Philippe; Costa, Bernard; Guezennec, Jean.
A bacterium isolated from microbial mats located on a polynesian atoll produced a high molecular weight (3,000 kDa) and highly sulphated exopolysaccharide. Previous studies showed that the chemical structure of this EPS consisted of neutral sugars, uronic acids, and high proportions of acetate and sulphate groups. The copper- and iron-binding ability of the purified pre-treated native EPS was investigated. Results showed that this EPS had a very high affinity for both copper (9.84 mmol g(-1) EPS) and ferrous iron (6.9 mmol g(-1) EPS). Amazingly, this EPS did not show any affinity for either ferric ions or selenium salts. This finding is one of the first steps in assessing the biotechnological potential of this polysaccharide.
Tipo: Text Palavras-chave: Sulphated exopolysaccharide; Microbial mats; Iron; Copper; Binding capacity.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6397.pdf
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Iron homeostasis related genes in rice Genet. Mol. Biol.
Gross,Jeferson; Stein,Ricardo José; Fett-Neto,Arthur Germano; Fett,Janette Palma.
Iron is essential for plants. However, excess iron is toxic, leading to oxidative stress and decreased productivity. Therefore, plants must use finely tuned mechanisms to keep iron homeostasis in each of their organs, tissues, cells and organelles. A few of the genes involved in iron homeostasis in plants have been identified recently, and we used some of their protein sequences as queries to look for corresponding genes in the rice (Oryza sativa) genome. We have assigned possible functions to thirty-nine new rice genes. Together with four previously reported sequences, we analyzed a total of forty-three genes belonging to five known protein families: eighteen YS (Yellow Stripe), two FRO (Fe3+-chelate reductase oxidase), thirteen ZIP (Zinc regulated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Rice; Genome analysis; Iron transporters; Ferritin.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400012
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Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats Anais da ABC (AABC)
SOUZA,JANAINA S. DE; BRUNETTO,ERIKA L.; NUNES,MARIA TEREZA.
ABSTRACT Iron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cardiac muscle; Iron; Myoglobin; Skeletal muscle.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602277
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Iron Transformation Pathways and Redox Micro-Environments in Seafloor Sulfide-Mineral Deposits: Spatially Resolved Fe XAS and delta Fe-57/54 Observations ArchiMer
Toner, Brandy M.; Rouxel, Olivier; Santelli, Cara M.; Bach, Wolfgang; Edwards, Katrina J..
Hydrothermal sulfide chimneys located along the global system of oceanic spreading centers are habitats for microbial life during active venting. Hydrothermally extinct, or inactive, sulfide deposits also host microbial communities at globally distributed sites. The main goal of this study is to describe Fe transformation pathways, through precipitation and oxidation-reduction (redox) reactions, and examine transformation products for signatures of biological activity using Fe mineralogy and stable isotope approaches. The study includes active and inactive sulfides from the East Pacific Rise 9 degrees 50'N vent field. First, the mineralogy of Fe(III)-bearing precipitates is investigated using microprobe X-ray absorption spectroscopy (RXAS) and X-ray...
Tipo: Text Palavras-chave: Hydrothermal; East Pacific Rise; X-ray absorption spectroscopy; Stable isotopes; Micro-environment; Mineral alteration; Iron; Biosignature.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00337/44810/44375.pdf
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Iron transportation across the placenta Anais da ABC (AABC)
Oliveira,Claudia M. de; Rodrigues,Marcio N.; Miglino,Maria Angélica.
According to the classification of placental types among animals, the transfer of iron through the placenta can occur via: absorption connected to transferin through the outer surface of the trophoblast in direct contact with circulating maternal blood; absorption of the erythrocytes by the chorionic epithelium in direct contact with accumulation of blood extravased from haemotophagous areas; absorption by the chorionic epithelium in direct contact with iron enriched secretions from the endometrial glands and absorption by extravasations of the blood in the maternal-fetal surface and the subsequent phagocytosis of the erythrocytes by trophoblast cells described in bovine, small ruminants, canine and feline. The function of erythrophagocytosis observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endocitosis; Iron; Phagocytosis; Placental hematoma.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000400025
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Iron Uptake by Fungi Isolated from Arcelor Mittal -Annaba- in the Northeast of Algeria Rev. Bras. Ciênc. Avic.
Bourzama,G; Ouled-Haddar,H; Marrouche,M; Aliouat,A.
ABSTRACT The Metal pollution is one of the major risks in the world nowadays. Iron is an essential metal for growth and proliferation of a vast majority of organisms, but it can be toxic to human health and other living beings in the environment at high concentrations due to its increased industrial activity. Fungi have a remarkable capacity to uptake and detoxify iron metal using different mechanisms such as bioaccumulation. Thus, the aim of this work is to study the ability of iron uptake by the fungal strains isolated from Arcelor Mittal -Annaba- in Algeria. Three strains were screened at high concentration of iron (1 g/l) and their capacity to uptake iron has been studied on Czapek Yeast Agar medium. The amounts of uptaken iron ions were estimated in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fungi; Iron; Screening; Toxic; Uptake.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000100313
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Iron-depletion prevents biofilm formation in Pseudomonas aeruginosa through twitching motility and quorum sensing BJM
Cai,Yun; Wang,Rui; An,Mao-Mao; Bei-Bei,Liang.
Influence of iron-depletion on twitching motility and quorum sensing (QS) system in P. aeruginosa was evaluated. The results demonstrated iron-depletion can retard biofilm formation and increase the twitching motility and expression of QS-related genes, suggesting a potential interaction between twitching motility and QS system in P. aeruginosa biofilm formation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Biofilm; Twitching motility; Quorum sensing.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100008
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Kinetics of iron (II) oxidation in seawater of various pH OMA
Roekens, E.J.; Van Grieken, R.E..
The rate of iron(II) oxidation in the North Sea water of pH 5.5-10 in the range 10-25°C has been studied. The oxygenation rate depends linearly on the iron(II) and dissolved oxygen concentrations. The second-order dependence on (OH<sup>-</sup>), found by several investigators for synthetic solutions, was confirmed in seawater, but only for pOH>6.9. For pOH<5.9 the rate appeared to be independent of the pOH. In the intermediate range, pOH<5.9-6.9, corresponding to the natural pH of seawater, a first-order dependence on the pOH is obeyed. The important discrepancy in the literature between the second-order rate constant for NaCHO<sub>3</sub> solutions and for seawater can be attributed predominantly to the incorrect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Kinetics; Oxidation.
Ano: 1983 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3293
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Linking ocean biogeochemical cycles and ecosystem structure and function: results of the complex SWAMCO-4 model ArchiMer
Pasquer, B; Laruelle, G; Becquevort, S; Schoemann, V; Goosse, H; Lancelot, C.
We present results obtained with SWAMCO-4, a complex model of the marine planktonic system calculating C, N, P, Si, Fe cycling within the upper ocean, the export production and the exchange of CO2 between the ocean and atmosphere. The model, constrained by physical, chemical and biological (grazing, lysis) controls, explicitly details the dynamics of four relevant phytoplankton functional groups with respect to C, N, P, Si, Fe cycling and climate change. Those are diatoms, pico/nano phytoplankton, coccolithophorids, and Phaeocystis spp. whose growth regulation by light, temperature and nutrients has been obtained based on a comprehensive analysis of literature reviews on these taxonomic groups. The performance of SWAMCO-4 is first evaluated in a 1D...
Tipo: Text Palavras-chave: Ecological modelling; Diatoms; Pico/nanophytoplankton; Coccolithophorids; Phaeocystis spp.; Iron; Air-sea CO2 fluxes; Global ocean; Antarctic ocean; North Atlantic.
Ano: 2005 URL: https://archimer.ifremer.fr/doc/00232/34302/32677.pdf
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Lipid peroxidation, antioxidant enzymes and glutathione levels in human erythrocytes exposed to colloidal iron hydroxide in vitro BJMBR
Ferreira,A.L.A.; Machado,P.E.A.; Matsubara,L.S..
The free form of the iron ion is one of the strongest oxidizing agents in the cellular environment. The effect of iron at different concentrations (0, 1, 5, 10, 50, and 100 µM Fe3+) on the normal human red blood cell (RBC) antioxidant system was evaluated in vitro by measuring total (GSH) and oxidized (GSSG) glutathione levels, and superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and reductase (GSH-Rd) activities. Membrane lipid peroxidation was assessed by measuring thiobarbituric acid reactive substance (TBARS). The RBC were incubated with colloidal iron hydroxide and phosphate-buffered saline, pH 7.45, at 37oC, for 60 min. For each assay, the results for the control group were: a) GSH = 3.52 ± 0.27 µM/g Hb; b) GSSG = 0.17 ± 0.03...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Superoxide dismutase; Glutathione; Iron; Erythrocyte; Thiobarbituric acid reactive substance.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000600004
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Long-Term In Situ Survey of Reactive Iron Concentrations at the EMSO-Azores Observatory ArchiMer
Laes-huon, Agathe; Cathalot, Cecile; Legrand, Julien; Tanguy, Virginie; Sarradin, Pierre-marie.
A study of the temporal dynamics of iron concentrations and temperature on a faunal assemblage at the Lucky Strike vent was performed using the Tempo ecological module at the EMSO-Azores deep-sea observatory. The CHEMINI in situ analyzer was implemented on this structure to determine reactive iron concentrations in unfiltered seawater samples along with a temperature probe. Stability tests were performed on the CHEMINI analyzer before deployment (optical module, hyperbaric tests, and deep-sea calibration) for long-term in situ analysis of reactive iron (six months, 2013–2014) at the Tour Eiffel active edifice. Recorded daily, the in situ standard (25 \mu mol.L {}^{-1} ) showed excellent reproducibility (1.07%, n=522 ), confirming satisfactory analytical...
Tipo: Text Palavras-chave: Deep-sea observatory; Hydrothermal vent; In situ chemical analyzer; Iron; Monitoring the Mid-Atlantic Ridge (MoMAR).
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00342/45275/46432.pdf
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Micronutrient concentration in potato clones with distinct physiological sensitivity to Al stress Ciência Rural
Tabaldi,Luciane Almeri; Castro,Gabriel Y; Cargnelutti,Denise; Skrebsky,Etiane Caldeira; Gonçalves,Jamile Fabbrin; Rauber,Renata; Rossato,Liana; Schetinger,Maria Rosa Chitolina; Bisognin,Dílson Antônio; Nicoloso,Fernando Teixeira.
The objective of this study was to evaluate the effects of aluminum (Al) on the zinc (Zn), manganese (Mn), iron (Fe) and copper (Cu) concentrations in four potato clones (Macaca and Dakota Rose: both Al-sensitive clones; and SMIC148-A and Solanum microdontum: both Al-tolerant-clones), grown in a nutrient solution (pH 4.00) with 0, 50, 100, 150 and 200mg Al L-1. Root Zn and Fe concentrations decreased linearly with the increase of Al levels in Macaca, SMIC148-A and Dakota Rose and increased linearly in S. microdontum. Shoot Zn concentration showed a quadratic relationship with Al in S. microdontum and SMIC148-A, but a curvilinear response in Dakota Rose. Shoot Fe concentration showed a quadratic relationship with Al in S. microdontum, SMIC148-A and Dakota...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solanum tuberosum; Solanum microdontum; Zinc; Manganese; Iron; Copper.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000200010
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Mitochondrial DNA damage associated with lipid peroxidation of the mitochondrial membrane induced by Fe2+-citrate Anais da ABC (AABC)
Almeida,Andréa M.; Bertoncini,Clélia R.A.; Borecký,Jiri; Souza-Pinto,Nadja C.; Vercesi,Aníbal E..
Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Iron; Lipid peroxidation; Mitochondria; MtDNA; Oxidative stress.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000300010
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Molecular and pathological basis of aceruloplasminemia Biol. Res.
KONO,SATOSHI; MIYAJIMA,HIROAKI.
Aceruloplasminemia is an autosomal recessive neurodegenerative disease characterized by iron accumulation in the brain as well as visceral organs. It is a loss-of-function disorder caused by mutations in the ceruloplasmin gene. Clinically, this disease consists of the triad of adult-onset neurological disease, retinal degeneration and diabetes mellitus. Massive iron accumulation and extensive loss of neurons are observed in the basal ganglia. The elevated iron concentration is associated with increased lipid peroxidation in the brains of aceruloplasminemia patients. Enlarged or deformed astrocytes and spheroid-like globular structures are characteristic neuropathological findings in aceruloplasminemia. Moreover, deformed astrocytes and globular structures...
Tipo: Journal article Palavras-chave: Ceruloplasmin; Iron; Lipid peroxidation; Mutation.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100003
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Nano partículas de hierro añadidas al oeste de las Islas Galápagos OceanDocs
Pazmiño, N..
La preocupación de efectuar estos estudios que alteran el medio natural, y que se dan en las aguas adyacentes a nuestra jurisdicción, pueden poner en riesgo la reserva marina de las Galápagos, por las condiciones oceanográficas en el área a 300 millas al Oeste de estas islas. Específicamente, el área influenciada por la Corriente Cromwell que fluye sub-superficialmente de Oeste a Este a través de la termoclina a lo largo de la línea ecuatorial puede afectar al ecosistema insular.
Tipo: Journal Contribution Palavras-chave: Iron; Chemical fertilizers; Environmental effects; Iron; Http://aims.fao.org/aos/agrovoc/c_3950.
Ano: 2007 URL: http://hdl.handle.net/1834/2365
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New insigths on the metabolic diversity among the epibiotic microbial communitiy of the hydrothermal shrimp Rimicaris exoculata ArchiMer
Zbinden, M; Shillito, B; Le Bris, Nadine; De Montlaur, C; Roussel, Erwan; Guyot, F; Gaill, F; Cambon-bonavita, Marie-anne.
The Rimicaris exoculata dominates the megafauna of some of the Mid Atlantic ridge hydrothermal vent sites. This species harbors a rich community of bacterial epibionts inside its gill chamber. Literature data indicate that a single 16S rRNA phylotype dominates this epibiotic community, and is assumed to be a sulfide-oxidizing bacteria. However attempts Of Cultivation were not successful and did not allow to confirm it. The aim of our study was to test the hypothesis of sulfide oxidation in the gill chamber, by a multidisciplinary approach, using in vivo experiments at in situ pressure in the presence of sulfide, microscopic observations and a molecular survey. Morphology of microorganisms, before and after treatment, was analyzed to test the effect of...
Tipo: Text Palavras-chave: Sulfur; Methane; Iron; Intracellular granules; Hydrothermal vent shrimp; High pressure experiments.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4563.pdf
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Notas acerca de la fertilización por hierro OceanDocs
Rentería, W.; Ortega, J..
La fertilización de los océanos con hierro han sido presentada como solución a corto plazo para poder reducir CO2 de la atmósfera. Esta afirmación presentada hace 20 años ha liderado y ha despertado un controversial debate sobre las posibles consecuencias en el clima. La bioquímica del hierro es un sistema complejo y su estudio en el océano es relativamente nuevo en comparación con los otros nutrientes, sin embargo, este sistema ha sido descrito en varios estudios que ayudan en la compresión de la afirmación intrincada sobre los beneficios de la fertilización con hierro. El objetivo de este estudio es describir los aspectos relacionados con los fundamentos de la fertilización con hierro, discutiendo acerca de la afirmación de la limitación de hierro y la...
Tipo: Journal Contribution Palavras-chave: Iron; Pollution effects; Carbon dioxide.
Ano: 2012 URL: http://hdl.handle.net/1834/4715
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On the early fate of hydrothermal iron at deep-sea vents: A reassessment after in situ filtration ArchiMer
Waeles, Mathieu; Cotte, Laura; Pernet-coudrier, Benoit; Chavagnac, V.; Cathalot, Cecile; Leleu, T.; Laes-huon, Agathe; Perhirin, Antoine; Riso, R. D.; Sarradin, Pierre-marie.
Deep-sea hydrothermal venting is now recognized as a major source of iron (Fe), an essential trace element that controls marine productivity. However, the reactions occurring during dispersal from buoyant plumes to neutrally buoyant hydrothermal plumes are still poorly constrained. Here we report for the first time on the dissolved-particulate partition of Fe after in situ filtration at the early stage of mixing at different hydrothermal discharges, i.e., Lucky Strike (37 degrees N), TAG (26 degrees N), and Snakepit (23 degrees N) on the Mid-Atlantic Ridge. We found that hydrothermal iron is almost completely preserved (>90%) in the dissolved fraction, arguing for low iron-bearing sulfide precipitation of iron in basalt-hosted systems with low Fe:H2S...
Tipo: Text Palavras-chave: Hydrothermal; Iron; Sulfide; Venting; Filtration; Precipitation.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00388/49944/50522.pdf
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